Table 2.
Proteins significantly reduced in sEV preparations obtained from MSCs cultured under hypoxic conditions
| Protein (Gene name) | Gene ID | Ratio |
|---|---|---|
| Inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4) | 3700 | 0.208 |
| Heparin cofactor-2 (SERPIND1) | 3053 | 0.280 |
| Alpha-2-HS glycoprotein (AHSG) | 197 | 0.301 |
| Kininogen-1 (KNG1) | 3827 | 0.409 |
| Lysyl oxidase homolog-4 (LOXL4) | 84,171 | 0.422 |
| Retinol-binding protein-4 (RBP4) | 5950 | 0.427 |
| Proteoglycan-4 (PRG4) | 10,216 | 0.443 |
| Signal peptide, CUB and EGF-like domain-containing protein-3 (SCUBE3) | 222,663 | 0.456 |
| Cholesteryl ester transfer protein (CETP) | 1071 | 0.482 |
| Secreted phosphoprotein-2 (SPP2) | 6694 | 0.495 |
| von Willebrand factor (VWF) | 7450 | 0.498 |
| Immunoglobulin heavy variable 4–28 (IGHV4-28) | 28,400 | 0.552 |
| Inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) | 3699 | 0.576 |
| Coiled-coil domain-containing protein-73 (CCDC73) | 493,860 | 0.595 |
| Mannan-binding lectin serine protease-2 (MASP2) | 10,747 | 0.598 |
| Lymphocyte antigen-6 complex locus protein G6f (LY6G6f) | 259,215 | 0.605 |
| Fibulin-1 (FBLN1) | 2192 | 0.606 |
| Peroxiredoxin-6 (PRDX6) | 9588 | 0.607 |
| Phosphatidylinositol-glycan-specific phospholipase D (GPLD1) | 2822 | 0.610 |
| Complement C1s subcomponent (C1S) | 716 | 0.614 |
| F-actin-capping protein subunit alpha-1 (CAPZA1) | 829 | 0.617 |
| Tyrosine protein kinase Lyn (LYN) | 4067 | 0.618 |
| Galectin-1 (LGALS1) | 3956 | 0.643 |
| Complement C1q tumor necrosis factor-related protein-3 (C1QTNF3) | 114,899 | 0.649 |
| Complement C1q subcomponent subunit-C (C1QC) | 714 | 0.651 |
| Kallistatin (SERPINA4) | 5267 | 0.657 |
| Haptoglobin (HP) | 3240 | 0.657 |
| Fibronectin (FN1) | 2335 | 0.659 |
| Procollagen C-endopeptidase enhancer-2 (PCOLCE2) | 26,577 | 0.662 |
| IgGFc-binding protein (FCGBP) | 8857 | 0.662 |
| Endoplasmin (HSP90B1) | 7184 | 0.664 |
| Carboxypeptidase N subunit-2 (CPN2) | 1370 | 0.667 |
| 6-phosphogluconate dehydrogenase, decarboxylating (PGD) | 5226 | 0.667 |
The column in the center presents gene IDs. The right column exhibits ratios of protein abundances in sEVs obtained from MSCs cultured under hypoxic conditions (1% O2; sEVhypoxic) and sEVs obtained from 41.5 MSCs cultured under regular ‘normoxic’ conditions (21% O2; sEVnormoxic) determined by LC/MS–MS