Fig. 5.

IGFBP-1 stimulation increases β1-integrin activation in human podocytes. Human podocytes were stimulated with IGFBP-1 (50 ng/ml, 15 min) before immunoprecipitation with antibodies specifically recognising conformationally active β1-integrin. Representative western blot for β1-integrin in both total cell lysates (‘Total’) and immunoprecipitated fractions (‘Active’), and densitometry representing the ratios of Active to Total β1-integrin, indicating a higher proportion of Active β1-integrin following IGFBP-1 stimulation, n = 7, *p < 0.05, t test