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. 2021 Apr 29;64(7):1626–1641. doi: 10.1007/s00125-021-05453-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — TLO detection in pancreas transplanted into recipients in whom recurrent type 1 diabetes developed. (a, b) Representative immunofluorescence images of pancreatic TLOs associated with islets in a biopsy sample (donor no. 3678). Triple immunofluorescence staining for CD3⁺ T cells, CD20⁺ B cells and collagen III for RFs (arrows) and the interstitial matrix of the islet and exocrine pancreas are shown (a). A parallel section was stained for PDGFrβ⁺ FRCs associated with the collagen III⁺ RFs (b); CD45 staining marks leucocytes. Boxed areas are shown at higher magnification. (c, d) Triple immunofluorescence staining using the same antibody combination as in (a) and (b) reveals TLO in the wall of the pancreatic duct. Boxed area is shown at higher magnification. (e, f) Triple staining for PLM, MECA79 and CD45 or CD31 revealing MECA79 staining of HEVs (arrowheads) and of some epithelial cells in the pancreatic ducts (arrows). Scale bars, 50 μm (a–c, e, f) or 25 μm (inset in b, d). Coll, collagen; du, duct; fo, follicle