Figure 4.

Secondary Measurements to Confirm Changes in Collagen Proteostasis, Including LOX Content (A, C), AGEs (B, D), and Protein Carbonylation (E). Protein solubility was performed in Gastroc followed by measurement of the collagen content of pepsin soluble fraction (F), pepsin insoluble fraction (G), total collagen content (H) by OHP assay, and collagen FSR of the soluble and insoluble muscle (I). TA collagen content (J), the ratio of collagen packing (K), and representative images of using polarized-light imaging picrosirius red staining (L). N = 5–12 muscles age group. Values expressed as mean ± SEM. Significance was determined by an unpaired t-test.