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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Biochem Pharmacol. 2021 Feb 3;189:114453. doi: 10.1016/j.bcp.2021.114453

Figure 4.

Figure 4.

In vitro cytotoxicity of PEI25K and EGFP/PEI25K polyplexes, PMet-P(cdmPEG2K) micelles and EGFP/PMet-P(cdmPEG2K) micelleplexes at various N/P ratios against 4T1.2 cells after 4 h incubation in FBS-free culture medium at pH 7.4 (A) and pH 6.8 (B) and after 48 h incubation in culture medium (pH 7.4) containing 10% FBS. In vitro cell viability profiles of DOX solution, PMet-P(cdmPEG2K) micelles, DOX-loaded PMet-P(cdmPEG2K) micelles, IL-12 loaded and IL-12/DOX co-loaded PMet-P(cdmPEG2K) micelleplexes against 4T1.2 cells after 72 h incubation in culture medium at pH 7.4 (C) and pH 6.8 (D). (E) In vitro cytotoxicity of DOX solution, PMet-P(cdmPEG2K) micelles, DOX-loaded PMet-P(cdmPEG2K) micelles, IL-12 loaded and IL-12/DOX co-loaded PMet-P(cdmPEG2K) micelleplexes against 4T1.2 cells after 72 h incubation in culture medium at pH 7.4 and pH 6.8 at a DOX concentration of 500 ng/mL.