Table 3:

Standardized Coefficients for FBS and TAGs Reported in Serum

TAG	Standardized Coefficients
	FA 14:0	FA 16:1	FA 16:0	FA 18:2	FA 18:1	FA 18:0	FA 20:2	FA 20:1
TAG 48:3	nd
TAG 48:2	nd	−0.64 p < 0.001	−0.48 p < 0.001	nd	−0.74 p < 0.001	nd	nd	nd
TAG 48:1	−0.18 p = 0.21	−0.15 p = 0.30	−0.28 p = 0.06	nd	−0.21 p = 0.15	nd	nd	nd
TAG 50:3	nd	nd	nd	nd	−0.65 p < 0.001	nd	nd	nd
TAG 50:2	nd	nd	nd	nd	−0.64 p < 0.001	nd	nd	nd
TAG 50:1	0.00 p = 1.00	0.02 p = 0.92	0.34 p = 0.02	nd	0.08 p = 0.61	0.49 p < 0.001	nd	nd
TAG 52:4	nd
TAG 52:3	nd	−0.60 p < 0.001	nd	−0.23 p = 0.05	nd	nd	nd	nd
TAG 52:2	nd	nd	nd	nd	−0.53 p < 0.001	nd	nd	nd
TAG 52:1	nd	nd	0.60 p < 0.001	nd	0.26 p =0.08	nd	nd	nd
TAG 54:4	nd	nd	nd	−0.44 p < 0.001	−0.41 p < 0.001	nd	nd	nd
TAG 54:2	nd	nd	−0.40 p < 0.01	nd	−0.20 p = 0.13	0.24 p = 0.05	nd	−0.31 p = 0.02
TAG 56:4	nd	nd	nd	nd	−0.40 p < 0.01	nd	nd	nd
TAG 56:3	nd	nd	nd	nd	−0.71 p < 0.001	nd	nd	−0.78 p < 0.001
TAG 56:2	nd	nd	nd	nd	−0.05 p = 0.76	0.41 p < 0.01	nd	−0.07 p = 0.63
TAG 56:1	nd

The standardized coefficients for FBS with respect to each serum-relevant TAG²⁹ were evaluated to determine their relationship. Standardized coefficients are a ratio of the amount of change in standard-deviation units for the outcome variable (here, each TAG) per corresponding change in the predictor variable (here, FBS). Positive values describe a positive association, while negative values describe a negative association. Multiple isomers were detected for each TAG provided in Brovkovych et al.²⁹ Fourteen of 19 isomers that reached significance were negatively associated with FBS, ruling out the possibility for FBS contamination or spillover into our extracted HMGEC lipidome. Bolded values denote significance.

FBS = fetal bovine serum

TAG = triacylglycerol

HMGEC = human meibomian gland epithelial cell