KEY RESOURCES TABLE
| REAGENT OR RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-P63 | Santa Cruz | Cat#sc-8343; RRID: AB_653763 |
| Mouse monoclonal anti-P63 | Biocare Medical | Cat# CM163; RRID: AB_10583039 |
| Chicken polyclonal anti-GFP | Aves Labs | Cat#GFP-1020; RRID: AB_2307323 |
| Rabbit polyclonal anti-Sox9 | Millipore | Cat#AB5535; RRID: AB_2239761 |
| Goat polyclonal anti-tdTomato | Biorbyt | Cat#orb182397; RRID: AB_2687917 |
| Mouse monoclonal anti-Foxj1 | eBioscience | Cat#14-9965-82; RRID: AB_1548835 |
| Rabbit polyclonal anti-pMek1/2 | Cell Signaling Technology | Cat#2338S; RRID: AB_490903 |
| Rabbit polyclonal anti-pErk1/2 | Cell Signaling Technology | Cat#4370S; RRID: AB_2315112 |
| Rabbit polyclonal anti-pP38 | Cell Signaling Technology | Cat#9215S; RRID: AB_331762 |
| Rabbit polyclonal anti-pJNK | Cell Signaling Technology | Cat#9251S; RRID: AB_331659 |
| Rabbit polyclonal anti-Kdr | Cell Signaling Technology | Cat#9698S; RRID: AB_11178792 |
| Mouse monoclonal anti-Muc5AC | Lab Vision | Cat#MS-145-P0; RRID: AB_62735 |
| Rabbit polyclonal anti-Clca3 | Abcam | Cat#ab46512; RRID: AB_152837 |
| Rabbit polyclonal anti-Scgb1a1 | Millipore | Cat#07-623; RRID: AB_310759 |
| Rat monoclonal anti-human SCGB1A1 | R&D Systems | Cat#MAB4218; RRID: AB_394324 |
| Mouse monoclonal anti-Acetylated tubulin | Sigma-Aldrich | Cat#T7451; RRID: AB_ 609894 |
| Rat monoclonal APC anti-mouse Epcam | Biolegend | Cat#118213; RRID: AB_1134105 |
| Mouse monoclonal APC anti-human p75 | Biolegend | Cat#345107; RRID: AB_ 10639737 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| PneumaCult-Ex Plus Medium | StemCell Technologies | Cat#05008 |
| PneumaCult-ALI Medium | StemCell Technologies | Cat#05001 |
| Collagen Type IV | Sigma-Aldrich | Cat#234154 |
| Collagenase Type I | Gibco | Cat#17100-017 |
| Elastase | Worthington Biochemical Corporation | Cat#LS002279 |
| Dispase II | BD Biosciences | Cat#354235 |
| Dnase I | Roche | Cat#10104159001 |
| Naphthalene | Sigma-Aldrich | Cat#84679 |
| HDM | Greer Laboratories | Cat#XPB70D3A25 |
| Tamoxifen | Sigma-Aldrich | Cat#T5648 |
| LIVE/DEAD Fixable Violet Dead Cell Stain Kit | Thermo Fisher Scientific | Cat#L34963 |
| Semaxanib | Selleck | Cat#S2845 |
| PD98059 | Selleck | Cat#S1177 |
| FR180204 | Selleck | Cat#S7524 |
| SB203580 | Selleck | Cat#S1076 |
| SP600125 | Selleck | Cat#S1460 |
| RNAscope 2.5 HD Assay-RED Kit | Advanced Cell Diagnostics | Cat#322360 |
| mm-Kdr probe | Advanced Cell Diagnostics | Cat#414811 |
| mm-Vegfa probe | Advanced Cell Diagnostics | Cat#405131 |
| hs-KDR probe | Advanced Cell Diagnostics | Cat#312121 |
| hs-Vegfa probe | Advanced Cell Diagnostics | Cat#423161 |
| PPIB probe | Advanced Cell Diagnostics | Cat#313901 |
| DapB probe | Advanced Cell Diagnostics | Cat#310043 |
| Deposited data | ||
| Single cell RNA sequencing data of Normal and NAPH treated lung epithelium | This paper | GEO: GSE# |
| Experimental Models: Cell Lines | ||
| Primary Human Small Airway Epithelial Cells | ATCC | PCS-301-010 |
| Experimental Models: Organisms/Strains | ||
| Shh-Cre | (Harfe et al., 2004) | N/A |
| Scgb1a1-CreER | (Rawlins et al., 2009) | N/A |
| Sox9-CreER | (Soeda et al., 2010) | N/A |
| Mek1loxp/loxp | (Bissonauth et al., 2006) | N/A |
| Mek2−/− | (Belanger et al., 2003) | N/A |
| Kdr-GFP | Jackson Laboratory | 017006 |
| Kdr-Cre | Jackson Laboratory | 018976 |
| Kdrloxp/loxp | Jackson Laboratory | 018977 |
| Sox9loxp/loxp | Jackson Laboratory | 013106 |
| R26tdTomato | Jackson Laboratory | 007914 |
| R26lacZ | Jackson Laboratory | 003474 |
| Vegf-hyper | (Miquerol et al., 1999) | N/A |
| Vegf-hypo | (Damert et al., 2002) | N/A |
| Vegfloxp/loxp | Kindly provided by Dr. Hoon-Ki Sung | N/A |
| Oligonucleotides | ||
| Primer: Mouse Kdr Forward: CGAGACCATTGAAGTGACTTGCC | This paper | N/A |
| Primer: Mouse Kdr Reverse: TTCCTCACCCTGCGGATAGTCA | This paper | N/A |
| Primer: Mouse Scgb1a1 Forward: GGTTATGTGGCATCCCTGAAGC | This paper | N/A |
| Primer: Mouse Scgb1a1 Reverse: GCTTACACAGAGGACTTGTTAGG | This paper | N/A |
| Primer: Mouse Vegfa Forward: CTGCTGTAACGATGAAGCCCTG | This paper | N/A |
| Primer: Mouse Vegfa Reverse: GCTGTAGGAAGCTCATCTCTCC | This paper | N/A |
| Primer: Human Kdr Forward: GGAACCTCACTATCCGCAGAGT | This paper | N/A |
| Primer: Human Kdr Reverse: CCAAGTTCGTCTTTTCCTGGGC | This paper | N/A |
| Primer: Human p63 Forward: CAGGAAGACAGAGTGTGCTGGT | This paper | N/A |
| Primer: Human p63 Reverse: AATTGGACGGCGGTTCATCCCT | This paper | N/A |
| Primer: Human Scgb1a1 Forward: TCATGGACACACCCTCCAGTTATGAG | This paper | N/A |
| Primer: Human Scgb1a1 Reverse: TGAGCTTAATGATGCTTTCTCTGGGC | This paper | N/A |
| Primer: Human Foxj1 Forward: GGCATAAGCGCAAACAGCCG | This paper | N/A |
| Primer: Human Foxj1 Reverse: TCGAAGATGGCCTCCCAGTCAAA | This paper | N/A |
| Primer: Human Sox9 Forward: AGGAAGCTCGCGGACCAGTAC | This paper | N/A |
| Primer: Human Sox9 Reverse: GGTGGTCCTTCTTGTGCTGCAC | This paper | N/A |
| Primer: Human Muc5AC Forward: GCACCAACGACAGGAAGGATGAG | This paper | N/A |
| Primer: Human Muc5AC Reverse: CACGTTCCAGAGGCCGGACAT | This paper | N/A |
| Software and Algorithms | ||
| Prism 6 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| R | Bioconductor | https://www.bioconductor.org/ |