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. 2021 Jun 8;12:3469. doi: 10.1038/s41467-021-23686-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Eluted material of cytosolic (c) or membrane (m) fractions used for immunoprecipitation from H26 or HTQ236 (sepF::sepF-ha) were loaded on 15% SDS-gels a and analyzed via Western blots using antibodies either against FtsZ1 or FtsZ2 b. As control purified FtsZ1 or FtsZ2 were loaded. The HA-tagged SepF was visible on the Coomassie-stained gels in all pulldown fractions of strain HTQ236 (indicated by the black arrows). Experiments were repeated three times. c Fluorescence microscopy of SepF-GFP in ftsZ1, ftsZ2, and ftsZ1/ftsZ2 deletion strains. d Fluorescence microscopy of MTS_SepF-GFP in ftsZ1, ftsZ2, and ftsZ1/ftsZ2 deletion strains. The experiments were repeated three times independently with similar results. Scale bars: 4 µm.