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. 2021 Jun 8;12:3460. doi: 10.1038/s41467-021-23546-6

Fig. 3. Administration of VEGFC mRNA-LNPs induces local lymphatic proliferation in mice in vivo.

Fig. 3

a–b Assessment of lymphatic proliferation 5 days after intradermal injection with 1 µg of Poly(C) or VEGFC mRNA-LNPs. EdU staining and anti-LYVE1 immunostaining of slides processed by paraffin-based histology (bars, 50 µm) shown by widefield (a) and confocal imaging (b). Arrows indicate EdU and LYVE1 double positive lymphatic endothelial cell (LEC) nuclei. Representative images of 3 ears of 3 mice in each group are shown. c Number of EdU positive LECs (two-tailed, unpaired T-test, P = 0.0260 for 3 mice) and mitotic index (two-tailed, unpaired T-test, P = 0.0266 for 3 mice) are shown 5 days after intradermal injection with 1 µg of Poly(C) or VEGFC mRNA-LNPs. Quantitative data for lymphatic proliferation are represented as mean and SEM from 3 injected ears of 3 mice in each group.