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Multiplex RPA primer and probe locations developed to specifically detect genus Clavibacter (A) and C. nebraskensis (B). (A) Target fragment size 129 bp, reverse primer labeled with DIG; (B) Target fragment size 156 bp, reverse primer labeled with FAM. The probe and primer are 49- and 32-bp long, respectively. THF-tetrahydrofuran residue; DIG-digoxigenin; FAM—6-carboxy-fluorescein.
