Table 2.
Details of multiplex RPA primers and probes developed for specific detection of Clavibacter in general and C. nebraskensis.
| Name | Sequences | Length (bp) | Product size (bp) | Target |
|---|---|---|---|---|
| C-RPA-F1 | 5′-AGCGCGTCCGACTCAGAGGTCCTGGGCAAAGC-3′ | 32 | 129 | Clavibacter |
| C-RPA-R1 | 5′/DigN/CTGCCGCCGAACCTCTGGGTGTACGGGTTCAT-3′ | 32 | ||
| C-RPA-P | 5′/Biosg/TCCAGCTTCTTGAAGACGAGCTGCCCGAGC-dSpacer-GGAGGCTCAGGAGCGAGA/Spacer-3′ | 49 | ||
| Cn-RPA-F11 | 5′-TGCACCTTCATCACGACATGGCACCCGATGCG-3′ | 32 | 156 | C. nebraskensis |
| Cn-RPA-R11 | 5′/56-FAM/AGCACACGATGATCATGCTGGCAATGGCCGAT-3′ | 32 | ||
| Cn-RPA-P11 | 5′/Biosg/TCGTCATGAGCGGCCACCTCGCGGCCATGT-dSpacer-CGGCCTGAGTCCGCTGTT/Spacer-3′ | 49 |