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. 2021 Jun 8;12(6):594. doi: 10.1038/s41419-021-03874-7

Fig. 5. FGF2 secreted by fibroblasts is involved in G-MDSC-mediated suppression of senescence and apoptosis.

Fig. 5

A KEGG pathway enrichment showing signaling pathways related to G-MDSCs. B Heatmap showing the expression levels of genes involved in signaling by the FGFR pathway in the MDSC-treated mice and the control mice. Red indicates a positive correlation, and blue indicates a negative correlation. C The mRNA levels of FGF2 in the fibroblasts and G-MDSCs cultured alone or cocultured with each other for 24 h analyzed by qPCR; n = 6 per group. D LVEF, LVDs, LV dp/dTmax, and LV dp/dTmin of the mice administered G-MDSCs with or without BGJ398 (5 µm); n = 5 per group. E–H Senescent fibroblasts were cocultured with G-MDSCs for 24 h, and BGJ398 (5 µm) was used to neutralize FGF2. E The levels of β-galactosidase in fibroblasts analyzed by flow cytometry through C12FDG staining. Representative cytograms are shown on the left, and statistical data are shown on the right; n = 7 per group. F The mRNA levels of the senescence markers Cdkn2a and Cdkn2b in fibroblasts; n = 8 per group. G Representative images and statistical data of senescent fibroblasts using X-gal staining. Red arrows represent X-Gal+ fibroblasts; n = 7 per group. Scale bars, 50 μm. H Representative flow cytometric profile and statistical data of fibroblast apoptosis through Annexin V-FITC and PI staining; n = 7 per group. The data are presented as the means ± SDs. The data are presented as the means ± SDs. Differences were determined by one-way ANOVA (more than 2 groups), and Tukey’s HSD post hoc test was performed. *P < 0.05.