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. 2021 Jun 8;11:12039. doi: 10.1038/s41598-021-91362-5

Figure 3.

Figure 3

Surface phenotypes of monocytes and neutrophils after surgery indicate an increased number of immature cells in blood of HLM patients. Flow cytometry analysis of CD66b+ neutrophils (a) and CD14+ monocytes (b) from whole blood of HLM patients taken before cardiac surgery (pre-operative), immediately after surgery (post-operative) and one day after admission to intensive care (post-operative + 1d) are shown. (a) Immature phenotype of CD66b+ blood neutrophils was analyzed by surface expression of CD10, CD16 and CD62L. Representative zebra plots show a change of the surface phenotype by the shift of the population to the lower right quadrant for CD10 and CD16 and to the higher right quadrant for CD62L. (b) Surgery-induced changes on CD14+ blood monocytes are represented by zebra plots of HLA-DR and CD62L expression patterns. Left plots show proper gate setting defined by isotype control stainings.