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. 2021 Jun 8;11:12039. doi: 10.1038/s41598-021-91362-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in cytokine secretion and innate immune cell activation in whole blood from HLM patients after surgery. Blood samples from HLM patients were taken before cardiac surgery (pre-operative, non-filled bars), directly after surgery (post-operative, light grey bars) and one day after admission to intensive care (post-operative + 1d, dark grey bars) and either mock-infected, treated with C. albicans or S. aureus for 4 h. (a) Plasma levels of TNF-α, IL-1β, IL-6 and IL-10 were quantified and bars are shown as means ± SD. Results are presented as pg/ml; N/A stands for values not available. (b) Surface marker expression was analyzed on the total monocyte population and on pathogen-associated neutrophils by flow cytometry. Data shown are mean fluorescence intensity (MFI) ± SD. Significance is shown as *P < 0.05; **P < 0.01; ***P < 0.001, unpaired, two-sided Student t test.