Figure 4.

Recombinant human Hsp-70 decreases H₂O₂-induced airway epithelial cell apoptosis from TA in the controls and patients with BPD. (A) To detect the role of Hsp-70 in early apoptotic cells with annexin-V-positive but PI-negative respiratory epithelial cells from TA after exposure to hydrogen peroxide, a FITC annexin-V/propidium iodide Apoptosis Detection Kit I (BD Pharmingen, USA) was used. Recombinant human Hsp-70 (5 μg/ml) was incubated with primary epithelial cells (1 × 10⁵ cells) from the TA of patients with BPD and of control subjects and then cultured with H₂O₂ (0.5 mM) for 1 h followed by 8 h of recovery. Representative figures are shown. (B) Statistical data of the experiments with 10 paired samples. The Kruskal–Wallis test was used to determine significant differences. *p < 0.05 after treatment and ^#p < 0.05 compared to the control group.