Figure 4.

Circ_0068087 silencing attenuates ox-LDL-induced damage in HUVECs partly by upregulating miR-186-5p. (A–J) HUVECs were transfected with si-circ_0068087 alone or together with miR-186-5p inhibitor prior to ox-LDL exposure (40 mg/L; 24 h). One-way ANOVA followed by Tukey's post hoc test was used to assess the differences. (A) MTT assay was performed to analyze cell viability. This experiment was performed three times with five technical repetitions each time. (B) Cell apoptosis rate was assessed through performing flow cytometry. This experiment was performed three times with three technical repetitions each time. (C,D) The levels of proliferation marker (PCNA) and apoptosis marker (Bcl-2) were measured by Western blot assay. This experiment was performed three times. (E–G) Cell inflammatory response was evaluated by ELISA. This experiment was performed three times with three technical repetitions each time. (H–J) The levels of MDA and ROS and the activity of SOD were determined by their commercial kits. This experiment was performed three times with three technical repetitions each time. *P < 0.05.