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. 2021 Jun 8;4:703. doi: 10.1038/s42003-021-02204-z

Fig. 1. Body weight, behavioral phenotype, and mitochondrial function.

Fig. 1

Graphs show untransformed mean, SEM and individual data points (gray/white = wild-type mice, red/black = A226Y mice). ***p < 0.001, **p < 0.01, *p < 0.05, ~p < 0.1, ns p ≥ 0.1. a Body weight from 4 to 13 months of age (months F1,115 = 87.06, p < 0.0001, η² = 0.43; genotype F1,11 = 39.76, p < 0.0001, η² = 0.78; genotype × months F1,115 = 5.233, p = 0.0240, η² = 0.04, Box-Cox λ 0.0). b Forepaw grip strength, assessed during 2 subsequent test sessions of 5 trials (session F1,16 = 29.39, p < 0.0001, η² = 0.65; genotype F1,16 = 9.307, p = 0.0076, η² = 0.37; genotype × session F1,16 = 0.126 ns). c Time to fall off the accelerating rotarod, average of 5 trials (genotype F1,16 = 0.050 ns). d Average velocity during walking bouts in the large open-field arena (genotype F1,16 = 3.320, p = 0.0872, η² = 0.17). e Average acceleration during walking bouts in the large open-field arena (genotype F1,16 = 3.672, p = 0.0734, η² = 0.19, Box-Cox λ −0.5). f The oxygen consumption rate (OCR) was measured under different respiratory states induced by the sequential injection of ADP (to induce state 3), oligomycin (O, to induce state 4o), FCCP (to induce state 3 uncoupled), and rotenone/antimycin A (R/A, to shut down mitochondrial respiration). Data represent the mean and SEM of N = 8 (WT) and N = 10 (A226Y) with 2 replicates per animal. Statistical analysis: Two-way ANOVA, multiple comparison between means of WT and A226Y over time (excluding the last time point after R/A injection that is an internal control of the experiment), p-value = 0.0063. g ATP levels. (13 ≤ N ≤ 20). h Detection of reactive oxygen species (ROS) production using MitoSOX (11 ≤ N ≤ 17). N = number of independent mice in each comparison.