Fig. 3. Protein degradation pathways in the muscle of Rps2 A226Y mutant mice at 15 months of age.

Graphs show mean, SEM and individual data points. ^***p < 0.001, ^**p < 0.01, ^*p < 0.05. a Heatmap representing the significantly upregulated genes (p < 0.05) in the KEGG pathway ‘Proteasome (mmu03050)’. b Heatmap representing the significantly upregulated genes (FDR < 0.05) involved in priming mitochondria for mitophagy³⁵. c Western blot and densitometry of total Parkin content in A226Y vs WT (N = 5). Tubulin as loading control. d Heatmap of Foxo3-regulated atrogenes³⁹, and a plot of –log10 FDR adjusted p-values for each Foxo3-regulated atrogene (N = 30) (A226Y vs WT) in 9 and 15 months mouse muscle. The dashed line indicates p = 0.05. e Western blot and densitometry of total and specific phosphorylated Foxo3 protein (N = 5). Tubulin as loading control. Relative quantity of phosphorylated protein calculated as phosphorylated/total. N = number of independent mice in each comparison.