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. 2020 Oct 27;13:11. doi: 10.1007/s40820-020-00537-8

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© The Author(s) 2020, corrected publication 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 13 — Combined effect of NPs with light and US. a Scheme illustrating the synergy proposed. b Cell viability after incubation with the proposed nanosystem (B-TiO_2−x-PEG). c Cell viability after different treatments. ***p < 0.001. d Flow cytometry apoptosis assay after different treatments, staining with Annexin-FITC and PI. e Confocal laser scanning microscopy images of after different treatments, PI (red fluorescence), calcein-AM (green fluorescence). Scale bar 40 μm. f Confocal laser scanning microscopy images of cells stained with 2′–7′dichlorofluorescein diacetate after different treatments, scale bar 20 μm. g Flow cytometry measurement of ROS production. Adapted with permission from [154].

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