Figure 1.

Schematic illustrating the strategy used to identify individual mutants in pooled DNA samples. Primers were designed using sequence data for the A. phagocytophilum strain HGE1 genome, and information from Illumina sequencing to identify transposon insertion sites. Two sets of primers were used to amplify across the 5’ and 3’ junctions between the transposon and genomic sequences, and a third set was used to amplify DNA across both flanking regions and the transposon.