Skip to main content
. 2021 Jun 2;79(5):ftab029. doi: 10.1093/femspd/ftab029

Figure 4.

Figure 4.

Screen shots from the Artemis genome browser, depicting selected genomic regions of A. phagocytophilum strain HGE1. (A) A large hypothetical gene (HGE1_03162/APH_0720) with multiple transposon insertions, encoding a 3038 amino acid protein that is expressed in tick cell culture. Red boxes indicate unique insert sites, and yellow boxes indicate insertions that may be located in other genomic regions with identical flanking sequences. (B) Components of the Type IV secretion system (T4SS) are spared from insertions with one exception of an insertion into the 3’ region of the virB6-4 gene (HGE1_01722/APH_0377; not shown, see text). The ORFs at HGE1_01665/APH_0371 encoding superoxide dismutase, HGE1_01675/APH_0373 encoding type IV secretion system ATPase VirB4-1 and HGE1_01680/APH_0374 to HGE1_01690/APH_0376 encoding type IV secretion system proteins VirB6-1 to VirB6-3 are depicted. C. The operon encoding multiple ribosomal proteins from HGE1_01235/APH_0281 encoding rplD 50S ribosomal protein L4 to HGE1_01330/APH_0299 secY preprotein translocase subunit SecY are devoid of transposon insertions. Shown are the reading frames on separate lines, with black vertical bars indicating stop codons. In panel A, only the forward reading frame is shown. In panels B and C, the two center horizontal bars contain the annotation for the forward or the reverse strand. Transposon insertion sites are annotated in red or yellow on the forward annotation track (in A), while protein coding sequences (CDS) are depicted in blue on the reading frame track and the gene is annotated in white on the annotation track.