Fig. 8. FcγRIIb and LilrB2 promote formation of shorter, more numerous Aβ fibrils.

Aβ-Cy5 monomer (20 µM) was polymerized for 24 h under control conditions, or in the presence of calmodulin (0.1 and 0.5 µM), FcγRIIb (1, 2, and 5 µM), or LilrB2 (0.2, 1, and 2 µM). a–c, g–i, and m–o show SIM images of the resulting fibrils. Scale bars are 2 µm. d, j, and p show mean fibril length under each condition. e, k, and q show cumulative distributions of fibril length; the insets indicate the number of fibrils larger than 2 µm. f, l, and r show the number of fibrils/μm². Data represent mean ± S.E. and ***P < 0.001 (two-sided Student’s t-test). n.s., not statistically significant. Numbers of biological independent samples (N) and analyzed images (X) in each condition are: N > 3 and X > 10, respectively.