Table 1.
Primers used for the identification and/or genetic characterization of Rickettsia species infecting ticks collected in this study from small ruminants.
| Assays | Target genes | Primers | Sequences (5′-3′) | Amplicon size (bp) | References |
|---|---|---|---|---|---|
| Single PCRa | |||||
| 16S rRNA | TQ16S+1F | CTGCTCAATGATTTTTTAAATTGCTGTGG | 324 | (22) | |
| TQ16S-2R | ACGCTGTTATCCCTAGAG | ||||
| Nested PCRb | |||||
| First PCR | ompB | rompB_OF | GTAACCGGAAGTAATCGTTTCGTAA | 511 | (23) |
| rompB OR | GCTTTATAACCAGCTAAACCACC | ||||
| Second PCR | rompB_SFG_IF | GTTTAATACGTGCTGCTAACCAA | 425 | ||
| rompB SFG-IR | GGTTTGGCCCATATACCATAAG | ||||
| Semi-nested PCRc | |||||
| First PCR | ompA | Rr190.70p | ATGGCGAATATTTCTCCAAAA | 631 | (24) |
| Rr190.701n | GTTCCGTTAATGGCAGCATCT | ||||
| Second PCR | Rr190.70p | ATGGCGAATATTTCTCCAAAA | 532 | ||
| Rr190.602n | AGTGCAGCATTCGCTCCCCCT | ||||
| Single PCRc | |||||
| gltA | RpCS.877p | GGGGGCCTGCTCACGGCGG | 381 | (25) | |
| RpCS.1258n | ATTGCAAAAAGTACAGTGAACA |
a
Single PCR based on the 16S rRNA gene allowing the selection of tick samples with DNA extraction efficiency.
b
Nested PCR based on the ompB gene allowing the detection and/or characterization after sequencing of Rickettsia species.
c
Single and semi-nested PCR based on gltA and ompA genes, respectively, allowing the characterization after sequencing of Rickettsia species.