TABLE 1.

Kinetic parameters for WT and A198W TS.

Assay	Protein	k cat (s−1)	K M,IGP (mM)	K M,indole (μM)	k cat/K M (M−1s−1)	(k cat/K M)A198W/(k cat/K M)WT
αTS alone	WT	(2.88 ± 0.54) × 10–4	0.94 ± 0.28		0.31 ± 0.11
	A198W	(3.81 ± 10.16) × 10–4	1.31 ± 0.09		0.29 ± 0.02	0.94 ± 0.34
TS complex	WT	2.83 ± 0.24	0.49 ± 0.10		(5.8 ± 1.2) × 103
	A198W	4.14 ± 0.25	0.42 ± 0.06		(9.9 ± 1.4) × 103	1.7 ± 0.4
βTS assay	WT	3.81 ± 0.38		25 ± 5	(1.5 ± 0.3) × 105	-
	A198W	3.08 ± 0.26		16 ± 4	(1.9 ± 0.5) × 105	1.3 ± 0.4