Figure 1.

Extracellular nucleic acids presence in the ECM of C. albicans biofilm. (A) 10⁵ cells/ml or 10⁷ cells/ml of C. albicans were placed in a 96-well plate in 100 μl RPMI 1640 and cultured aerobically for 48 hours at 37°C. The formed biofilms were stained with Sytox Green (a final concentration of 1 μM) and imaged using fluorescence microscopy in the green channel and transmitted light (AE31E, Motic microscope). (B) C. albicans (ATCC 10231, SC5413) cells present in hyphal form in 48 hours-biofilm (10⁵ cells/ml) were visualized with an Olympus IX73 microscope.