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. 2021 May 26;12:667782. doi: 10.3389/fmicb.2021.667782

FIGURE 6.

FIGURE 6

The effect of fecpEC41 on the growth of A487 under iron-limited conditions and in urine and on the ability of the bacteria to colonize urinary tracts. (A) The growth of A487/pEC41 and A487/pEC41Δfec cultured in ILM-CA. (B) The growth of A487/pEC41 and A487/pEC41Δfec cultured in ILM-CA supplemented with 100 μM ferric citrate. (C) The growth of A487/pEC41 and A487/pEC41Δfec cocultured in urine. The results are shown as the relative growth rates compared to A487/pEC41 at each indicated time point. For (A–C), the results are presented as the means ± SD. The data are representative of three independent experiments performed in triplicate. (D) Cochallenge of A487/pEC41 and A487/pEC41Δfec in a mouse model of UTI. Equal numbers of the two strains were transurethrally coinoculated into female C3H/HeN mice (N = 15). At 24 h postinfection, the bacterial counts and the CI of A487/pEC41Δfec vs. A487/pEC41 in bladders and kidneys were determined. The horizontal bars indicate median values. The dashed line represents the log10 scale of CI = 1. The two-way ANOVA was used in the statistical analysis of (A,B). The paired two-tailed student’s t-test was used in the statistical analysis of (C), while the Wilcoxon signed-rank test was used in (D). *P < 0.05, **P < 0.01, ***P < 0.001.