TABLE 1.
Bacterial strains and plasmids used in this study.
| Strains or plasmids | Description | Known AR markersa | Sources |
| Strains | |||
| EC41 | An E. coli strain isolated from the blood of a patient | Ap, Str, Km | This study |
| A487 | An E. coli strain isolated from the urine of a patient with cystitis | Mao et al., 2012 | |
| CFT073str | A spontaneous streptomycin-resistant mutant of the urosepsis-causing E. coli strain CFT073 | Str | This study |
| UTI89str | A spontaneous streptomycin-resistant mutant of the cystitis-causing E. coli strain UTI89 | Str | This study |
| MG1655str | A spontaneous streptomycin-resistant mutant of E. coli K12 MG1655 | Str | This study |
| IP10 | An entA mutant of MG1655 with an insertion of a kanamycin resistance cassette in the entA locus | Km | This study |
| MP10 | An entA mutant of MG1655str | Str | This study |
| MP10LA | A lacZ mutant of MP10 with an insertion of a chloramphenicol resistance cassette in the lacZ locus | Str, Cm | This study |
| ELA41 | MP10LA harboring pEC41 | Str, Cm, Ap | This study |
| IP10/pEC41 | IP10 harboring pEC41 | Km, Ap | This study |
| MP10/pEC41 | MP10 harboring pEC41 | Str, Ap | This study |
| Δfec-MP10 | A fecMG1655 deletion mutant of MP10 with an insertion of a gentamicin resistance cassette in the chromosomal fecMG1655 locus | Str, Gm | This study |
| MP10/pEC41Δfec | MP10 harboring pEC41Δfec | Str, Ap, Cm | This study |
| Δfec-MP10/pEC41 | Δfec-MP10 harboring pEC41 | Str, Ap, Gm | This study |
| Δfec-MP10/pEC41Δfec | Δfec-MP10 harboring pEC41Δfec | Str, Ap, Gm, Cm | This study |
| ΔfecpEC41-EC41 | A fecEC41 deletion mutant of EC41 with an insertion of chloramphenicol resistance cassette in the fecEC41 locus | Ap, Cm | This study |
| A487/pEC41 | A487 harboring pEC41 | Ap | This study |
| A487/pEC41Δfec | A487 harboring pEC41Δfec | Ap, Cm | This study |
| CFT073str/pEC41 | A spontaneous streptomycin-resistant strain of E. coli CFT073 harboring pEC41 | Str, Ap | This study |
| UTI89str/pEC41 | A spontaneous streptomycin-resistant strain of E. coli UTI89 harboring pEC41 | Str, Ap | This study |
| Plasmids | |||
| pEC41 | The plasmid isolated from EC41 | Ap | This study |
| pEC41Δfec | A fecEC41 deletion mutant of pEC41 with an insertion of chloramphenicol resistance cassette in the fecEC41 locus | Ap, Cm | This study |
aAR markers, antimicrobial resistance markers commonly used in laboratories; Ap, ampicillin; Str, streptomycin; Km, kanamycin; Cm, chloramphenicol; Gm, gentamicin.