Fig. 2.

Effects of differing extracellular matrix extraction methods on uniform decellularized protein pellet.A, workflow of ECM extraction (blue) and single-shot (purple) methods used. All ECM methods were preceded by the same decellularization. B, total collagen PSMs identified by each method. Multistep extraction (1’) is shown as both a sum of the two fractions and the average PSMs per fraction. Plotted as average values with SD. For Gnd-HCl/HA method, light blue indicates Gnd-HCl fraction, darker blue indicates HA fraction. C, exclusive unique collagen peptides identified by each method. Numbers on bars represent the number of distinct collagen chains identified with a minimum of two peptides. D, Bar graph of glycoprotein and proteoglycan PSMs by each method. Multistep extraction (1’) shown as both a sum of two fractions and the average PSMs per fraction. E, exclusive unique peptides identified for glycoproteins and proteoglycans by each method. Numbers on bars represent the number of glycoproteins and proteoglycans identified with a minimum of two peptides.