Table 1.
Sequences of primers used in this study
| Target genes |
Primer sequence (5′-3′) | Annealing temperature (°C) |
References |
| blaSHV | F: GGTTATGCGTTATATTCGCC R: TTAGCGTTGCTTGTGCTC |
50 | (20) |
| blaTEM | F: ATGAGTATTCAACATTTCCG R: CTGACAGTTACCAATGCTTA |
50 | (21) |
| blaVEB-1 | F: CGACTTCCATTTCCCGATGC R: GGACTCTGCAACAAATACGC |
55 | (22) |
| blaPER-1 | F: AATTTGGGCTTAGGGCAGAA R: ATGAATGTCATTATAAAAGC |
53 | (23) |
| blaGES-1 | F: ATGCGCTTCATTCACGCAC R: CTATTTGTCCGTGCTCAGG |
53 | (24) |
| blaOXA-4 | F: TCAACAGATATCTCTACTG TT R: TTTATCCCATTTGAATATGGT |
50 | (25) |
|
blaOXA-10 (group I) |
F: AGCCGTTAAAATTAAGCCC R: CTTGATTGAAGGGTTGGGCG |
56 | (22) |
|
blaOXA-2 (group II) |
F: GCCAAAGGCACGATAGTTGT R: GCGTCCGAGTTGACTGCCGG |
62 | (26) |
|
blaOXA-1 (group III) |
F: AGCCGTTAAAATTAAGCCC R: CTTGATTGAAGGGTTGGGCG |
53 | (27) |
| blaCTX-M-1 | F: ATGGTTAAAAAATCACTGCGTC R: TTGGTGACGATTTTAGCCGC |
55 | (28) |
| blaCTX-M-2 | F: ATGATGACTCAGAGCATTCG R: TGGGTTACGATTTTCGCCGC |
55 | (28) |
| blaCTX-M-8 | F: ACTTCAGCCACACGGATTCA R: CGAGTACGTCACGACGACTT |
55 | (28) |
| blaCTX-M-9 | F: ATGGTGACAAAGAGAGTGCA R: CCCTTCGGCGATGATTCTC |
55 | (28) |
| rep-PCR | F: IIIGCGCCGICATCAGGC R: ACGTCTTATCAGGCCTAC |
45 | (29) |