Skip to main content
. 2021 Mar 24;8(11):2004856. doi: 10.1002/advs.202004856

Figure 5.

Figure 5

A) Representative bright‐field and fluorescent micrographs to evaluate morphology, intracellular esterase‐activity, and hypoxia during cultivation of spheroids in the microfluidic array. Scale bar, 1 mm. B) Morphometric analysis of area, perimeter, roundness, and solidity of A549, HepG2, Caco‐2, and NHDF spheroids with different sizes, n = 3 ± SD. Statistical analysis was performed using the one‐way ANOVA (*p < 0.0332, **p < 0.0021, ***p < 0002, ****p < 0.0001). C) Calcein and D) Hypoxia fluorescence intensities of the four cell lines at day 3 and 12 postseeding on‐chip of different sizes, as indicated in each graph, n = 3 ± SD. Statistical analysis was performed using one‐way ANOVA and Holm‐Sidak's multiple comparisons test (*p < 0.0332, **p < 0.0021, ***p < 0.0002, ****p < 0.0001).