Table 3.

Linear regression analysis and goodness‐of‐fit (R ²) values of generated sizes after 3 d postseeding of A549, HepG2, Caco‐2, and NHDF spheroids in respect to initial seeding densities Statistical significance of respective slopes was determined by analysis of covariance (ANCOVA). Data are expressed as mean value ± SD for n = 6. Underlined values are considered as the most optimal seeding density

	Seeding density [cells mL−1]
Cell line	1.0 × 105	2.5 × 105	5.0 × 105	7.5 × 105	1.0 × 106	3.0 × 106	p‐value of slopes
A549	0.8336	0.8388	0.8251	0.8725	0.8938	0.8747	P < 0.0001
HepG2	0.2701	0.4697	0.8278	0.6739	0.7988	0.8345	P < 0.0001
Caco‐2	0.1977	0.7552	0.7915	0.8140	0.9175	0.9662	P < 0.0001
NHDF	0.3463	0.3968	0.5596	0.5502	0.7430	0.7935	P = 0.1387