Table 4.

Linear regression analysis and goodness‐of‐fit (R ²) values of generated sizes of BBB spheroids after six days postseeding, including human primary astrocytes (hA), human primary pericytes (hP), and immortalized hCMEC/D3 (BEC) in a ratio of 1:1:3 in respect to initial seeding densities. Statistical significance of respective slopes was determined by analysis of covariance (ANCOVA). Data are expressed as mean value ± SD for n = 6. Underlined values are considered as the most optimal seeding density

	Seeding densities [cells mL−1]
BBB triple‐culture	1.0 × 106	2.0 × 106	3.0 × 106	5 × 106	p‐value of slopes
hA:hP:BEC (1:1:3)	0.9071	0.8389	0.9096	0.9318	P = 0.0009