Table 5.
Efficient Permeability P e of BBB spheroids of different sizes and cell ratios after 1 and 4 h of cultivation with 10 × 10−6 m FD4. Data are expressed as mean value ± SD for n = 6
| Efficient permeability P e (10–6 cm s−1) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1000 µm | 900 µm | 700 µm | 500 µm | 300 µm | ||||||
| BBB seeding ratio (hA:hP:BEC) | 1 h | 4 h | 1 h | 4 h | 1 h | 4 h | 1 h | 4 h | 1 h | 4 h |
| 1:1:3 | 3.3 ± 0.6 | 7.8 ± 1.3 | 4.5 ± 1.1 | 8.3 ± 1.1 | 4.9 ± 2.0 | 8.9 ± 2.3 | 5.2 ± 0.5 | 9.1 ± 1.3 | 6.2 ± 2.9 | 10.6 ± 2.3 |
| 1:1:2 | 6.0 ± 0.5 | 8.8 ± 1.4 | 7.5 ± 1.6 | 9.9 ± 1.4 | 8.1 ± 2.8 | 10.0 ± 1.8 | 8.9 ± 1.1 | 11.3 ± 2.1 | 9.9 ± 2.4 | 11.4 ± 3.0 |
| 1:1:1 | 7.0 ± 2.2 | 11.5 ± 2.1 | 9.2 ± 1.4 | 12.7 ± 1.4 | 9.6 ± 1.8 | 14.2 ± 1.4 | 10.4 ± 2.3 | 13.3 ± 2.8 | 11.1 ± 3.7 | 13.5 ± 1.2 |
| 5.5:1.5:3 | 2.9 ± 0.7 | 7.1 ± 1.5 | 4.4 ± 1.0 | 8.3 ± 1.9 | 5.1 ± 1.3 | 8.7 ± 2.2 | 4.6 ± 0.7 | 9.5 ± 2.3 | 5.4 ± 2.0 | 11.2 ± 1.1 |
| 1:0:0 | 11.5 ± 3.0 | 12.7 ± 1.9 | 11.8 ± 3.3 | 13.8 ± 3.3 | 12.1 ± 2.4 | 14.6 ± 2.9 | 12.4 ± 3.4 | 15.3 ± 2.6 | 14.1 ± 3.0 | 16.9 ± 3.6 |