Figure 5.

Differential effects of detergent‐induced pores in neutral versus charged GUVs in the presence Triton X‐100 at final concentration of 1 × 10⁻³ m. A) On neutral POPC GUVs, Triton X‐100 induces large area increase and formation of visible pores that last dozens of seconds before the vesicle is slowly solubilized (see arrowheads in the enhanced cropped images in the second row). B–D) In sharp contrast, on negative POPC:POPG GUVs the formation and rapid expansion of a single macropore (see arrows and encircled pore region in the middle image in C) proceeds to vesicle bursting strongly resembling response to electroporation. The process is viewed under (B) phase contrast, this sequence is shown in Movie S6 of the Supporting Information. (C) Epifluorescence, Movie S7 of the Supporting Information. (D) Confocal cross‐sections where the outside medium contains 2.5 × 10⁻⁶ m SRB, Movie S8 of the Supporting Information. E) In the presence of CaCl₂ (3.5 × 10⁻³ m), the macropore (indicated with arrows) stays open during the whole solubilization process, Movie S9 of the Supporting Information. The GUVs in the fluorescence images contain 0.5 mol% DPPE‐Rh. All time stamps correspond to the beginning of observation. Scale bars: 20 µm.