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. 2021 Apr 7;8(11):2004263. doi: 10.1002/advs.202004263

Figure 3.

Figure 3

Protocol for the establishment of pH‐stable Avidin–Alexa Flour 488 loaded polymersome B (Avidin–Alexa Fluor 488–Psome B(AAF‐Psome B)) through sequential pH‐dependent shear‐force‐driven hollow‐fiber‐filtration (HFF). The avidin locations: 1 (lumen), 2 (inner hydrophilic shell of membrane), 3 (hydrophobic membrane), 4 (membrane‐integrated avidin in the outer hydrophilic shell of membrane), and 5 (free). Loading efficiency (%) of different HFF purified AAF‐Psome B samples (right bottom: HFF B1, HFF B1‐2, and HFF B1‐3) calculated from the fluorescence spectra. pH‐dependent DLS–titration of AAF‐Psome B (HFF B1) (right) for validating collapsed membrane, starting of swelling membrane, and highly swollen membrane states for AAF‐Psome B (HFF B1) during different HFF purification processes at pH 7.0, 6.0, and 5.0, respectively.