FIGURE 1.

Lidocaine induces ferroptosis of ovarian and breast cancer cells. (A,B) SKOV-3 and T47D cells were treated with lidocaine at the indicated concentrations. The mRNA expression of SLC7A11 was analyzed by qPCR. (C,D) SKOV-3 and T47D cells were co-treated with erastin (5 mmol/L) and lidocaine (3 mM). The cell viability was detected by MTT assays after 48 h of the treatment. (E–H) SKOV-3 and T47D cells were treated with lidocaine (3 mM). The Fe²⁺ (E), iron (Tesfay et al., 2019), and lipid ROS levels (G) were detected. (H) The expression of GPX4 and SLC7A11 was measured by Western blot analysis. The results were quantified using ImageJ software. mean ± SD, **p < 0.05, **p < 0.01. The experiments were performed independently three times.