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. 2021 May 26;9:624553. doi: 10.3389/fbioe.2021.624553

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Copyright © 2021 Myram, Venzac, Lapin, Battistella, Cayrac, Cinquin, Cavaniol, Gropplero, Bonnet, Demolombe, Descroix and Coscoy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PCT Pkd1^+/- and Pkd1^-/- tube deformation in chips with 200 μm spacing. (A,B) Examples of temporal evolution of tubes with laminin coating, for Pkd1^+/- cells (A) and Pkd1^-/- cells (B). Scale bar:100 μm. Days after seeding: (A) 11, 16 (confluency), 20, (B) 9, 10 (confluency), 14. (C–E) Quantitative analysis, n = 12 Pkd1^+/- chips (black) and n = 14 Pkd1^-/- chips (red), all coatings pooled (see Supplementary Figure 8 for separated behavior). (C) Mean cell density over time. Error bars: S.E.M. (D) Kinetic evolution of mean tubes diameter normalized by diameters at seeding, in function of the time after tube confluency. A blue horizontal line at D/D₀ = 1, corresponding to no change in diameter, is indicated. Each time point corresponds to 8–35 tubes for Pkd1^-/-, 9–20 tubes for Pkd1^+/-. Error bars: S.E.M. (E) Maximum (over time) of the mean normalized diameter. Points correspond to individual tube values. Central bar, median; cross, mean; box, values between Q1 and Q3 quartiles; error bars, extreme values [between Q1 - 1.5*(Q3 - Q1) and Q3 + 1.5*(Q3 - Q1)]. (D,E) were computed only for tubes having reached full confluency during the observation period. ^∗∗ indicates statistically significant difference with p = 0.0002.