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. 2021 May 26;9:624553. doi: 10.3389/fbioe.2021.624553

FIGURE 6.

FIGURE 6

PCT Pkd1-/- tube deformation in chips with 100 μm spacing. (A) Example of temporal evolution of tubes seeded with Pkd1-/- cells at days 2, 4, 10, 17, 23, and 25 after seeding. Scale bar:100 μm. (B,C) The behavior in 100 μm spacing tubes (yellow) was assessed with laminin coating and compared to the behavior in 200 μm spacing laminin-coated tubes (red). (B) Kinetic evolution of mean tube diameter normalized by diameter at seeding, in function of the time after tube confluency. A blue horizontal line at D/D0 = 1, corresponding to no change in diameter, is indicated. Each time point corresponds to 9–26 tubes for 100 μm spacing, 4–19 tubes for 200 μm spacing. Error bars: S.E.M. (C) Maximum (over time) of the mean normalized diameter. Points correspond to individual tube values. Central bar, median; cross, mean; box, values between Q1 and Q3 quartiles; error bars, extreme values [between Q1 - 1.5*(Q3 - Q1) and Q3 + 1.5*(Q3 - Q1)]. (B,C) were computed only for tubes having reached full confluency during the observation period. ** indicates statistically significant difference with p = 0.001.