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. 2021 May 26;9:689613. doi: 10.3389/fcell.2021.689613

FIGURE 1.

FIGURE 1

Elevated CXCR2 and CXCL8 co-expression levels in NPC correlate with poor prognosis. (A) Representative immunohistochemistry images showing CXCR2 expression in stromal and tumor cells in different individual NPC tissue (left) and CXCL8 expression in other NPC tissues (right). Magnification, 20×. (B) Correlation analysis between CXCR2 and CXCL8. CXCL8 expression and CXCR2 expression in tumor cells was significantly positively associated with CXCR2 expression in stromal cells, R = 0.43, p < 0.001 and R = 0.32, p < 0.01, but no association was found between CXCR2 expression in tumor cells and CXCL8 expression in stromal cells, R = 0.14, p > 0.05. Statistical analysis was performed using the Pearson’s correlation coefficient and linear regression. (C–E) According to IHC score, samples from NPC patients (n = 99) were divided into two groups based on low or high expression of the indicated markers, including CXCR2 in tumor and stromal cell and CXCL8. Overall survival was significantly negatively associated with the levels of CXCR2 in tumor cells, CXCR2 in stromal cells and CXCL8, as displayed in Kaplan–Meier survival curves of OS, (C) p = 0.038; (D) p = 0.045; and (E) p = 0.026. (F–G) CXCR2 expression analysis of six NPC cell lines using flow cytometry and western blot. (IHC score, immunohistochemical analysis score; R, Spearman’s correlation).