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. 2021 May 26;9:689613. doi: 10.3389/fcell.2021.689613

FIGURE 3.

FIGURE 3

SB225002 sensitizes NPC cells to radiation in vitro. (A) SB225002 sensitized C666-1 and HONE-1 cells to radiation. Cells were treated with 0.5 μM SB225002 and irradiated with doses as indicated. The colonies were counted 12 days later. Values represent mean ± SD (n = 3; *p < 0.05; **p < 0.01; Student’s t-test). (B,C) DSB repair assay was performed by counting γ-H2AX foci. C666-1 and HONE-1 cells pre-treated with SB225002 for 3-4 h were irradiated with 8 Gy and harvested to immunofluorescence stain with DAPI and FITC for γ-H2AX at 1 h later. (C) Representative immunostaining images were shown, 20 × oil. (B) Cells with more than 10 foci were scored as positive and plotted with histograms. Data are shown with the mean ± SD of n = 5-8 fields obtained from three parallel experiments (*p < 0.05; ***p < 0.001; Student’s t-test). (D) Cell cycle distribution analyzed by flow cytometry of PI staining. C666-1 and HONE-1 cells pre-treated with SB225002 for 3-4 h were irradiated with 8 Gy and harvested to analyze by flow cytometry 4 h later (n = 3; ***p < 0.001; Student’s t-test).