Skip to main content
. 2021 May 26;9:689613. doi: 10.3389/fcell.2021.689613

FIGURE 6.

FIGURE 6

Regulation of tumor angiogenesis by CXCR2 inhibitor. (A) Paraffin-embedded C666-1 tumor sections after SB225002 combined radiotherapy treatment were examined by immunohistochemical analysis with anti-CD31 and anti-VEGF antibody. Representative tumor vasculature from five groups was shown. (B) Representative photographs of HUVECs migration in vitro. HUVECs were seeded into the upper chamber of a transwell system, and tumor culture medium supernatant (TS) of nasopharyngeal carcinoma cell C666-1, human recombinant protein CXCL8 or SB225002 (0.5 μM), was added to the bottom chamber. Scale bar = 100 μm. (C) Statistical analysis of the number of migrated HUVECs. Data were assessed by ImageJ, showing as Mean ± SD (n = 3; ***p < 0.001; ###p < 0.001. * represent p value between tumor supernatant or CXCL8 group and serum free medium group, # represent p value between groups with or without SB225002. (D) Representative photographs of HUVEC tubule formation in vitro grown on the Matrigel after 3-4 h in the presence of tumor supernatant, human recombinant protein CXCL8 (50ng/ml) or SB225002(0.5 μM), compared to free serum medium. Scale bar = 500 μm. (E) Statistical analysis of junctions, tubules and total tubule length formed by HUVECs. Data were assessed by ImageJ showing as Mean ± SD (n = 3; *p < 0.05; **p < 0.01; ###p < 0.001. * represent p value between tumor supernatant or CXCL8 group and serum free medium group, # represent p value between groups with or without SB225002.