Bacterial strains |
|
Lactobacillus rhamnosus GG |
ATCC |
Cat# 53103 |
Lactobacillus acidophilus |
ATCC |
Cat# 4356 |
Lactobacillus bulgaricus LB-87 |
Custom Probiotic Inc |
Cat# CP-2016 |
Lactobacillus casei LC-11 |
Custom Probiotic Inc |
Cat# CP-2012 |
Lactobacillus salivarius LS-33 |
Custom Probiotic Inc |
Cat# CP-2010 |
Bacteroides thetaiotaomicron |
ATCC |
Cat# 29148 |
Bacteroides fragilis |
ATCC |
Cat# 25285 |
Eubacterium rectale |
ATCC |
Cat# 33656 |
E. coli Top10 |
Invitrogen |
Cat# C404006 |
E. coli BL21 (DE3) |
Invitrogen |
Cat# C600003 |
|
Plasmids and primers |
|
pET28b-MBP-TEV |
Addgene |
Cat# 69929 |
pET28b-MBP-TEV-Msp1 |
This study |
N/A |
pET28b-MBP-TEV-Msp2 |
This study |
N/A |
pET28b-MBP-TEV-Msp3 |
This study |
N/A |
Primers see Table S5
|
N/A |
N/A |
|
Chemicals, Peptides, and Recombinant Proteins |
|
MRS broth |
Hardy Diagnostics |
Cat# C5931 |
BHI broth |
Hardy Diagnostics |
Cat# C5141 |
Lemon fruits |
Sam’s Club |
Cat#127308 |
Kanamycin |
Sigma |
Cat# K1876 |
RNAprotect Bacteria Reagent |
Qiagen |
Cat# 76506 |
Porcine bile extract |
Sigma |
Cat# B8631 |
Ni-NTA Agarose |
Qiagen |
Cat# 30210 |
IRDye 800cw streptavidin |
VWR |
Cat# 102673-342 |
Dynabeads™ M-270 Streptavidin |
Invitrogen |
Cat# 65305 |
Tskgel G5000PW column |
VWR |
Cat# 100368-036 |
SuperScrip III First-Strand Synthesis System |
Invitrogen |
Cat#18080051 |
RNase-free DNase І |
NEB |
Cat# M0303S |
TEV protease |
Sigma |
Cat# T4455 |
Nuclease P1 |
NEB |
Cat# M0660S |
CIP |
NEB |
Cat# M0525S |
|
Critical Commercial Assays |
|
RNeasy Mini Kit |
Qiagen |
Cat# 74104 |
miRNeasy Mini Kit |
Qiagen |
Cat# 217004 |
RiboPure RNA Purification Kit, bacteria |
Invitrogen |
Cat# AM1925 |
QuantiTect SYBR Green PCR Kits |
Qiagen |
Cat# 204143 |
PKH26 Fluorescent Cell Linker Kit |
Sigma |
Cat# PKH26GL |
GeneArt Seamless Cloning and Assembly Kit |
Invitrogen |
cat# A14606 |
HiScribe T7 Quick High Yield RNA Synthesis Kit |
NEB |
Cat# E2050S |
QIAamp DNA Stool Mini Kits |
Qiagen |
Cat# 51504 |
|
Experimental Models: Organisms/Strains Mouse |
|
C57BL/6 |
Jackson Laboratory Stock |
Cat# 000664 |
|
Deposited data |
|
Sequenced data reported in this paper is stored at Sequence Read Archive (SRA): Accession code for Deposited Data: PRJNA722680 |
|
Software and algorithms |
|
Prism (GraphPad Software) |
GraphPad |
https://www.graphpad.com |
BioEdit |
BioEdit |
https://www.bioedit.com |
Sequence manipulation suite |
(Stothard, 2000) |
https://www.bioinformatics.org/sms2 |