Figure 4.

Sex bias in bile acid effects on gut transit (See also Figures S3,S4, and S7 and Table 1)

(A) Sex bias in transit times following intra-colonic bile acid infusions. In order to normalize recorded transit times (Table S4) to a single value, each recorded transit time was subtracted from the mean transit time of males after intra-colonic infusion with the buffer-only negative control.

(B) Sex-biased transit time differences in mice colonized with BSH-high consortia but not in mice harboring BSH-low consortia or SPF microbiota (including conventionally raised mice; transit times measured following intra-colonic infusion of PBS included), or in GF controls.

Statistical significance was determined using a Student's two-tailed t-test; ∗p< 0.05. In (A) and (B), the horizontal lines within boxes denote the mean values, while lower and upper boundaries of boxes represent the 25th and 75th percentiles, respectively, and whiskers represent 1.5 times the interquartile range.