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. 2021 Apr 15;30(12):1067–1083. doi: 10.1093/hmg/ddab104

Figure 5 .

Figure 5

Novel object recognition with mice transplanted with Ube3a lentivector transduced HSPC: (A,B) Newborn Ube3a-deficient IL2rg-/y mice were transplanted with either nontransduced (NT-HET) or Ube3a vector-transduced (Ube3a-HET) human CD34+ HSPC. Wild type IL2rg-/y mice (WT) were used as a control. Eight weeks post-transplant, mice were assessed for learning and memory abilities using the novel object recognition test. (A) Ube3a-HET mice exhibited intact object recognition following a one-hour delay, similar to WT while, NT-HET mice did not spend more time with the novel object, exhibiting a lack of recognition memory. (B) All groups explored the two objects similarly during the familiarization phase. (C, D) Ube3a-deficient adult mice were transplanted with either nontransduced (NT-HET) or Ube3a vector-transduced (Ube3a-HET) human CD34+ HSPC. Wild type IL2rg-/y mice (WT) were used as a control. Six weeks post i.v. injection in adult mice, subjects were assessed for learning and memory abilities using the novel object recognition test. (C) Ube3a-HET mice exhibited intact object recognition following a one-hour delay, similar to WT while, NT-HET mice did not spend more time with the novel object, exhibiting a lack of recognition memory. (D) All groups explored the two objects similarly during the familiarization phase. *p < 0.05, novel versus familiar.