Figure 8 .

Engraftment and development of human immune cells in NRG mice: Human CD34+ HSPC were left NT or transduced with either the EGFP control (EGFP) or Ube3a-expressing (hAS8) lentiviral vector. Cells were transplanted into 2–5-day-old NRG mice. At 16 weeks post-transplant, mice were euthanized, and human T cells were analyzed for CD3, CD4, and CD8 expression in the (A) peripheral blood, (B) spleen, and (C) thymus. Human B cells were analyzed for CD45 and CD19 in the (D) spleen and (E) bone marrow. (F) Human macrophages (CD45/CD14) and human CD34+ HSPC (CD45/CD34) were analyzed in the bone marrow.