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. 2021 Jun 9;2(3):100635. doi: 10.1016/j.xpro.2021.100635

Figure 5.

Figure 5

Crystals of the pHLA complex grown in 20% PEG3350 w/v, 0.2 M Potassium Formate, 1 mM CaCl2 via sitting-drop, vapor diffusion at 20°C with a protein: reservoir drop ratio of 1:1, at a protein concentration of 5 mg/mL

(A) Crystals appeared in 48 h.

(B) Optimization of crystals using micro-seeds from drop (A). Crystals started forming in 24 h.