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. 2021 Jun 9;2(3):100635. doi: 10.1016/j.xpro.2021.100635
REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and virus strains
BL21 (DE3) E. coli chemically competent cells Sigma-Aldrich Cat# CMC0014
Chemicals, peptides, and recombinant proteins
Additive Screen HT Hampton Research Cat# HR2-428
Agar Astral Scientific Cat# BIOFB0010
Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A7906
Bromophenol blue Sigma-Aldrich Cat# B0126
Deoxyribonuclease I from bovine pancreas (DNase I) Sigma-Aldrich Cat# DN25
Di-sodium ethylenediaminetetraacetic acid (EDTA) Sigma-Aldrich Cat# E5134
Dithiothreitol (DTT) GoldBio Cat# DTT10
Glutathione, Reduced GoldBio Cat# G-155-500
Glutathione, Oxidized GoldBio Cat# G-060-25
Glycerol Sigma-Aldrich Cat# G5516
Guanidine hydrochloride Sigma-Aldrich Cat# G3272
Isopropyl β-d-1-thiogalactopyranoside (IPTG) GoldBio Cat# 12481C100
Kanamycin monosulfate GoldBio Cat# K-120-50
L-Arginine monohydrochloride Sigma-Aldrich Cat# A5131
Lysozyme GoldBio Cat# L-040-25
Magnesium Chloride (MgCl2) Merck Cat# 1058330250
PEG/Ion HT Crystallization reagent kit Hampton Research Cat# HR2-139
Phenylmethylsulfonyl fluoride (PMSF) GoldBio Cat# P470
Polyethylene glycol 3350 (PEG3350) Sigma-Aldrich Cat# 88276
Potassium formate Merck Cat# 294454
SARS-CoV-2 Nucleocapsid protein-derived peptide N222-230 LLLDRLNQL GenScript N/A
Sodium chloride (NaCl) Merck Cat# 7647-14-5
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) prestained protein marker (10–180 kDa) Bio-Rad Cat# 1610394
Tris Thermo Fisher Cat# FSBBP152-5
Triton X-100 Sigma-Aldrich Cat# X100-1L
Tryptone Thermo Fisher Cat# LP0042R
Urea Thermo Fisher Cat# AJA817-5KG
Yeast Extract Sigma-Aldrich Cat# 09182-5KG-F
Recombinant DNA
pET-30a(+) vector encoding HLA-A∗02:01 heavy chain GenScript N/A
pET-30a(+) vector encoding β2-microglobulin GenScript N/A
Other
Acrodisk syringe filters, 0.22 um Pall Cat#4187
Autoclave N/A N/A
Cell spreaders N/A N/A
Cellulose acetate filter papers Sartorius Cat#11104—47----N
Centrifuge concentration filters 10 kDa cut-off Merck Cat# 900000072879
Crystal Clear Sealing Tape Hampton Research HR4-511
Crystal crusher Hampton Research HR4-216
Crystal plate fridge 20°C N/A N/A
Dialysis buckets, 15L N/A N/A
Dialysis membranes Livingstone Cat# DIALYSIS
Diethylaminoethyl (DEAE) Sepharose Fast Flow anion exchange chromatography resin Cytiva Cat# 17070910
Econo-Column Chromatography Columns Bio-Rad Cat# 7374251
Erlenmeyer Flasks, 2 Liters Duran Cat# 2121663
FPLC Bio-Rad NGC Chromatography System Quest 10
Fraction collection tubes for FPLC N/A N/A
Glass microfiber filters MC Scientific Cat# FF0483-047
High speed centrifuge Beckman Coulter Avanti J-E
HiTrap Q HP anion exchange chromatography column Cytiva Cat# 29-0513-25
Homogenizer Hanchen Lab Homogenizer AD200L-H
LAQUA-Twin pH 22 Meter Kit Westlab Cat# 3999960123
Magnetic flea N/A N/A
Microscope Leica N/A
MRC Maxi 48-well sitting drop crystallization plates Hampton Research HR3-179
NanoDrop 2000 Thermo Scientific N/A
Petri dishes, 90 mm Techno Plas Cat# S9014UV20
Protein Electrophoresis Equipment Bio-Rad PowerPac Basic
Schott bottles, plasticware (measuring cylinders, beakers) N/A N/A
Shaker incubators New Brunswick Innova 43
Solvac Filtering device Pall Cat#4020
Stirrer N/A N/A
Tube rotator Thermo Scientific 88881004
Water bath N/A N/A