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. 2021 May 7;24(6):102521. doi: 10.1016/j.isci.2021.102521

Figure 6.

Figure 6

CAN inhibited mTOR phosphorylation by binding to the FRB domain within mTOR

(A) Affinity chromatography results of CAN.

(B–D) (B) Protein identified using western blot analysis in (A). Molecular docking of (C) RAPA and (D) CAN binding to the FRB domain within mTOR.

(E) Common binding site of the FRB domain within mTOR to RAPA and CAN.

(F) Comparison of the predicted Gibbs free energy of RAPA and CAN binding to the FRB domain of mTOR (n = 5). RAPA, rapamycin; CAN, canagliflozin; NS, no significant difference; FRB, FKBP-RAPA-binding.