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. 2021 May 7;24(6):102521. doi: 10.1016/j.isci.2021.102521

Figure 7.

Figure 7

Protective effects of the mTOR inhibitor, RAPA, and the HIF-1a inhibitor LW6 on PA-induced HL-1 cell viability and inflammation

(A–E) (A) Cell viability was detected using an MTT assay in the RAPA-treated HL-1 cells (n = 3). mRNA expression levels of (B) Il-1a, (C) Il-6, (D) Tnf-a, and (E) Hif-1a in the RAPA-treated HL-1 cells (n = 3).

(F–J) (F) Cell viability was detected using an MTT assay in the LW6-treated HL-1 cells (n = 3). mRNA expression levels of (G) Il-1a, (H) Il-6, (I) Tnf-a, and (J) Hif-1a in the LW6-treated HL-1 cells (n = 3).

(K and L) (L) Protein expression levels of HIF-1a in RAPA and LW6-treated HL-1cells was determined using ImageJ software following western blot analysis (K). Data are presented as mean ± SD. #p < 0.05 and ##p < 0.01 versus ctrl; ∗p < 0.05 and ∗∗p < 0.01 versus Mod. Ctrl, untreated normal control; Mod, PA (0.25 mM)-treated control group; 100, HL-1 cells were treated with PA (0.25 mM) and RAPA (100 nM); 200, HL-1 cells were treated with PA (0.25 mM) and RAPA (200 nM); 400, HL-1 cells were treated with PA (0.25 mM) and RAPA (400 nM); 5, HL-1 cells were treated with PA (0.25 mM) and LW6 (5 μM); 10, HL-1 cells were treated with PA (0.25 mM) and LW6 (10 μM); 20, HL-1 cells were treated with PA (0.25 mM) and LW6 (20 μM).