Figure 4.

Propionate contributes to Sod2 propionylation at 132 lysine site in zebrafish fed high-fat diet

(A) HPLC-MS/MS spectra of an Sod2 peptide bearing propionylation (DFGSFQK_+57.0901MN).

(B) A representative western blotting showing patterns of Sod2 expression and Sod2 propionylation at the K132 and quantification of intestinal Sod2 protein level in zebrafish fed LFD, HFD, or HFSP0.5 for 2 weeks (n = 3).

(C) A representative western blotting showing patterns of Sod2 expression and Sod2 propionylation at the K132 in ZF4 cells treated with OPA or OPP.

(D) Western blotting showing the pattern of his-Sod2 acetylation purified from OPA- or OPP-treated HEK293 cells (n = 3).

(E) A representative western blotting showing the pattern of his-Sod2 propionylation at K132 incubated with the indicated concentrations of propionyl-CoA.

(F) The mRNA expression of genes encoding subunits of intestinal Pcc, an enzyme catalyzing the carboxylation of propionyl-CoA, in zebrafish fed HFD or HFSP0.5 for 2 weeks (n = 6).

Values are means ± SEM. A and D were analyzed by Student's t test, ∗, p < 0.05, NS, not significant. B and F were analyzed by Duncan's test and means without a common letter are significantly different (p < 0.05).

See also Figure S4.